Subacute stage of ishemic stroke (6-21 days) was characterized by demarcation increase of lesion contours. Bioplasm nls showed distinct bounded zones of infarction. Chromogeneity of these zones was still high (5-6 points according to Fleindler’s scale). Accompanied by hyperchromogeneity Bioplasm nls showed small segments of moderate chromogenic signal caused by increased protein contents.

Some necrotic patches began demonstrating clear boundaries in ischemic stroke organization stage (> 21 days) owing to edema absorption.

Demarcation due to gliosis began developing around necrosis lesion. Affection zone on Bioplasm nls usually decreased in size and gained sharp contours. About 6 weeks later necrotic masses were completely reabsorbed and replaced by glious tissue (or cyst was developing). Bioplasm nls displayed gliosis as increased chromogeneity zone, while cyst had achromogenic structure caused by liquor fluid.